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Immunohistochemical staining of human cerebral cortex, endometrium, eye, retina and liver using Anti-PDE6A antibody HPA016970 (A) shows similar protein distribution across tissues to independent antibody HPA074677 (B).
Immunohistochemical staining of human cerebral cortex, endometrium, eye, retina and liver using Anti-PDE6A antibody HPA016970 (A) shows similar protein distribution across tissues to independent antibody HPA074677 (B).
Immunohistochemical staining of human cerebral cortex, endometrium, eye, retina and liver using Anti-PDE6A antibody HPA016970 (A) shows similar protein distribution across tissues to independent antibody HPA074677 (B).

Anti-PDE6A-25ul

HPA016970
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDE6A-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16499
  • Protein Name
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
  • Scientific Description
    Rabbit Polyclonal Anti-PDE6A Antibody against Human phosphodiesterase 6A, cGMP-specific, rod, alpha. Validated for Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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MSDS

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Figure 1. Western blot analysis of PDE6 alpha/PDE6A using anti-PDE6 alpha/PDE6A antibody (A07800-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE6 alpha/PDE6A antigen affinity purified polyclonal antibody (Catalog # A07800-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE6 alpha/PDE6A at approximately 100KD. The expected band size for PDE6 alpha/PDE6A is at 100KD.
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Product group Antibodies
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Product group Antibodies
Atlas Antibodies
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