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Anti-PDE6B Antibody

144-06942
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDE6B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PDE6B Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5158
  • Target name
    PDE6B
  • Target description
    phosphodiesterase 6B
  • Target synonyms
    CSNB3, CSNBAD2, GMP-PDEbeta, PDEB, RP40, rd1, rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta, GMP-PDE beta, phosphodiesterase 6B, cGMP-specific, rod, beta, rod cGMP-phosphodiesterase beta-subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35913
  • Protein Name
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
  • Scientific Description
    PDE6B Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDE6 beta/PDE6B using anti-PDE6 beta/PDE6B antibody (A02659-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE6 beta/PDE6B antigen affinity purified polyclonal antibody (Catalog # A02659-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE6 beta/PDE6B at approximately 98KD. The expected band size for PDE6 beta/PDE6B is at 98KD.
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