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Western Blot (WB) analysis of HeLa cells using PDK1 Polyclonal antibody.
Western Blot (WB) analysis of HeLa cells using PDK1 Polyclonal antibody.
Western Blot (WB) analysis of HeLa cells using PDK1 Polyclonal antibody.

Anti-PDK1 Antibody

A01268
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDK1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PDK1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID5163
  • Target name
    PDK1
  • Target description
    pyruvate dehydrogenase kinase 1
  • Target synonyms
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial, PDH kinase 1, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15118
  • Protein Name
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
  • Scientific Description
    Boster Bio Anti-PDK1 Antibody catalog # A01268. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDK1 using anti-PDK1 antibody (A01268-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK1 antigen affinity purified polyclonal antibody (Catalog # A01268-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK1 at approximately 58 kDa. The expected band size for PDK1 is at 49 kDa.
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