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Figure 2. Immunocytochemistry staining of PDK1 using Anti-PDK1 Antibody (A01268-2). ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Figure 2. Immunocytochemistry staining of PDK1 using Anti-PDK1 Antibody (A01268-2). ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Figure 2. Immunocytochemistry staining of PDK1 using Anti-PDK1 Antibody (A01268-2). ICC staining PDK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

Anti-PDK1 Antibody

A01268-2
Boster Bio
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDK1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PDK1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID5163
  • Target name
    PDK1
  • Target description
    pyruvate dehydrogenase kinase 1
  • Target synonyms
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial, PDH kinase 1, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, pyruvate dehydrogenase kinase, isoenzyme 1
  • Host
    Rabbit
  • Protein IDQ15118
  • Protein Name
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
  • Scientific Description
    Boster Bio Anti-PDK1 Antibody catalog # A01268-2. Tested in WB,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of PDK1 using anti-PDK1 antibody (A01268-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK1 antigen affinity purified polyclonal antibody (Catalog # A01268-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK1 at approximately 58 kDa. The expected band size for PDK1 is at 49 kDa.
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