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anti-PDK2 antibody

ARG40833
Arigo Biolaboratories
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPDK2
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-PDK2 antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5164
  • Target name
    PDK2
  • Target description
    pyruvate dehydrogenase kinase 2
  • Target synonyms
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial; PDH kinase 2; PDHK2; PDKII; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase kinase, isozyme 2; pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDK2 using anti-PDK2 antibody (A03261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK2 antigen affinity purified polyclonal antibody (Catalog # A03261) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK2 at approximately 46KD. The expected band size for PDK2 is at 46KD.
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