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Western Blot analysis of HepG2 cells using PDK2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PDK2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using PDK2 Polyclonal Antibody

PDK2 Antibody

CSB-PA003732
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5164
  • Target name
    PDK2
  • Target description
    pyruvate dehydrogenase kinase 2
  • Target synonyms
    PDHK2, PDKII, pyruvate dehydrogenase kinase, isozyme 2, PDH kinase 2, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, pyruvate dehydrogenase kinase, isoenzyme 2, pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15119
  • Protein Name
    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDK2 using anti-PDK2 antibody (A03261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK2 antigen affinity purified polyclonal antibody (Catalog # A03261) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK2 at approximately 46KD. The expected band size for PDK2 is at 46KD.
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