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Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Anti-PDLIM7 Antibody

HPA018794
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDLIM7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDLIM7 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9260
  • Target name
    PDLIM7
  • Target description
    PDZ and LIM domain 7
  • Target synonyms
    LMP1, LMP3, PDZ and LIM domain protein 7, 1110003B01Rik, LIM domain protein, LMP, Lim mineralization protein 3, PDZ and LIM domain 7 (enigma), protein enigma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NR12
  • Protein Name
    PDZ and LIM domain protein 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PDLIM7 using anti-PDLIM7 antibody (A04377-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat skeletal muscle tisssue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse skeletal muscle tissue, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDLIM7 antigen affinity purified polyclonal antibody (Catalog # A04377-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDLIM7 at approximately 50 kDa. The expected band size for PDLIM7 is at 50 kDa.
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