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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Anti-PDPR Antibody

HPA048092
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPDPR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PDPR Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55066
  • Target name
    PDPR
  • Target description
    pyruvate dehydrogenase phosphatase regulatory subunit
  • Target synonyms
    PDP3, pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NCN5
  • Protein Name
    Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PDPR using anti-PDPR antibody (A12185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPR antigen affinity purified polyclonal antibody (Catalog # A12185) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPR at approximately 99 kDa. The expected band size for PDPR is at 99 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
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