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Anti-PEDF Antibody

144-64621
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSERPINF1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PEDF Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID5176
  • Target name
    SERPINF1
  • Target description
    serpin family F member 1
  • Target synonyms
    EPC-1, OI12, OI6, PEDF, PIG35, pigment epithelium-derived factor, alpha-2 antiplasmin, cell proliferation-inducing gene 35 protein, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1, testis tissue sperm-binding protein Li 70n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36955
  • Protein Name
    Pigment epithelium-derived factor
  • Scientific Description
    PEDF pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PEDF using anti-PEDF antibody (M02034). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEDF antigen affinity purified monoclonal antibody (Catalog # M02034) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEDF at approximately 50 kDa. The expected band size for PEDF is at 46,50 kDa.
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