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Immunohistochemical staining of human nasopharynx shows strong positivity in respiratory epithelial cells.
Immunohistochemical staining of human nasopharynx shows strong positivity in respiratory epithelial cells.
Immunohistochemical staining of human nasopharynx shows strong positivity in respiratory epithelial cells.

Anti-PELI2 Antibody

HPA053182
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPELI2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PELI2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57161
  • Target name
    PELI2
  • Target description
    pellino E3 ubiquitin protein ligase family member 2
  • Target synonyms
    E3 ubiquitin-protein ligase pellino homolog 2, RING-type E3 ubiquitin transferase pellino homolog 2, pellino homolog 2, pellino-2, protein pellino homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HAT8
  • Protein Name
    E3 ubiquitin-protein ligase pellino homolog 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PELI2 using anti-PELI2 antibody (A10176-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI2 antigen affinity purified polyclonal antibody (Catalog # A10176-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PELI2 at approximately 50 kDa. The expected band size for PELI2 is at 46 kDa.
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