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Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows cytoplasmic positivity in Purkinje cells.

Anti-PELI3 Antibody

HPA062281
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPELI3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PELI3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID246330
  • Target name
    PELI3
  • Target description
    pellino E3 ubiquitin protein ligase family member 3
  • Target synonyms
    E3 ubiquitin-protein ligase pellino homolog 3, RING-type E3 ubiquitin transferase pellino homolog 3, pellino homolog 3, protein pellino homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N2H9
  • Protein Name
    E3 ubiquitin-protein ligase pellino homolog 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PELI3 using anti-PELI3 antibody (A09622-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI3 antigen affinity purified polyclonal antibody (Catalog # A09622-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PELI3 at approximately 51 kDa. The expected band size for PELI3 is at 51 kDa.
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