Anti-PERV [7C4]

Absolute Antibody

Anti-PERV [7C4]

9

This antibody recognizes and binds an epitope between amino acids 517-537 on the p15E component of type A PERV Env protein.

This is a neutralizing antibody againt the porcine endogenous retrovirus. This antibody is unable to pull down the viral particle in an immunoprecipitation assay and has a relatively high IC50 in the neutralization assay which supports the notion that the neutralizing activity of 7C4 is due to the blockage of p15E fusogenic activity after the virus binds to the host cell receptor. Epitope mapping of the antibody was also done using Western blotting and verification of binding sites of the antibody was done by competition ELISA. It was reported that Env332-615 and Env460-539 recombinant proteins exhibited strong inhibition activity for 7C4 mAb binding to the immobilized antigen Env332-615 (Chiang et al., 2007).

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

7C4

Human

IgG1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203