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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.

Anti-PFKFB4 Antibody

HPA047719
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPFKFB4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PFKFB4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5210
  • Target name
    PFKFB4
  • Target description
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
  • Target synonyms
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6PF-2-K/Fru-2,6-P2ase 4, 6PF-2-K/Fru-2,6-P2ase testis-type isozyme, PFK/FBPase 4, bifunctional enzyme with kinase and biphosphatase activities
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16877
  • Protein Name
    6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PFKFB4 using anti-PFKFB4 antibody (A07028-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB4 antigen affinity purified polyclonal antibody (Catalog # A07028-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKFB4 at approximately 54 kDa. The expected band size for PFKFB4 is at 54 kDa.
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