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Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & vesicles.
Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & vesicles.
Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & vesicles.

Anti-PFN3 Antibody

HPA038274
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPFN3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PFN3 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID345456
  • Target name
    PFN3
  • Target description
    profilin 3
  • Target synonyms
    profilin-3, profilin-3, profilin III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60673
  • Protein Name
    Profilin-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PFN3 using anti-PFN3 antibody (A13729). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFN3 antigen affinity purified polyclonal antibody (Catalog # A13729) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFN3 at approximately 15 kDa. The expected band size for PFN3 is at 15 kDa.
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