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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts and in Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic  positivity in subset of cells in seminiferous ducts and in Leydig cells.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts and in Leydig cells.

Anti-PGAP1 Antibody

HPA069704
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetPGAP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PGAP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80055
  • Target name
    PGAP1
  • Target description
    post-GPI attachment to proteins inositol deacylase 1
  • Target synonyms
    Bst1; GPI inositol-deacylase; ISPD3024; MRT42; NEDDSBA; post-GPI attachment to proteins 1; post-GPI attachment to proteins factor 1; SPG67
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ75T13
  • Protein Name
    GPI inositol-deacylase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PGAP1 using anti-PGAP1 antibody (A08640). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGAP1 antigen affinity purified polyclonal antibody (Catalog # A08640) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGAP1 at approximately 100,58 kDa. The expected band size for PGAP1 is at 105,49-53,85-90 kDa.
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