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PGAM5 Antibody

LS-C501421
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPGAP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PGAM5 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80055
  • Target name
    PGAP1
  • Target description
    post-GPI attachment to proteins inositol deacylase 1
  • Target synonyms
    Bst1; GPI inositol-deacylase; ISPD3024; MRT42; NEDDSBA; post-GPI attachment to proteins 1; post-GPI attachment to proteins factor 1; SPG67
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PGAP1 using anti-PGAP1 antibody (A08640). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGAP1 antigen affinity purified polyclonal antibody (Catalog # A08640) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGAP1 at approximately 100,58 kDa. The expected band size for PGAP1 is at 105,49-53,85-90 kDa.
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