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Anti-PGM1 Antibody

144-06303
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetPGM1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PGM1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5236
  • Target name
    PGM1
  • Target description
    phosphoglucomutase 1
  • Target synonyms
    CDG1T; glucose phosphomutase 1; GSD14; PGM 1; phosphoglucomutase-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36871
  • Protein Name
    Phosphoglucomutase-1
  • Scientific Description
    PGM1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PGM1 using anti-PGM1 antibody (A02318-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat skin tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skin tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM1 antigen affinity purified polyclonal antibody (Catalog # A02318-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGM1 at approximately 64 kDa. The expected band size for PGM1 is at 61 kDa.
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