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Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Anti-PGM1 Antibody

HPA024637
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPGM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PGM1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5236
  • Target name
    PGM1
  • Target description
    phosphoglucomutase 1
  • Target synonyms
    CDG1T, GSD14, phosphoglucomutase-1, PGM 1, glucose phosphomutase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36871
  • Protein Name
    Phosphoglucomutase-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PGM1 using anti-PGM1 antibody (A02318-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat skin tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skin tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM1 antigen affinity purified polyclonal antibody (Catalog # A02318-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGM1 at approximately 64 kDa. The expected band size for PGM1 is at 61 kDa.
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