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Immunohistochemical staining of human prostate shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows cytoplasmic positivity in glandular cells.

Anti-PGM3 Antibody

HPA029760
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetPGM3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PGM3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5238
  • Target name
    PGM3
  • Target description
    phosphoglucomutase 3
  • Target synonyms
    AGM1, IMD23, PAGM, PGM 3, phosphoacetylglucosamine mutase, N-acetylglucosamine-phosphate mutase 1, acetylglucosamine phosphomutase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95394
  • Protein Name
    Phosphoacetylglucosamine mutase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PGM3 using anti-PGM3 antibody (A02052-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human k562 whole cell lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGM3 antigen affinity purified polyclonal antibody (Catalog # A02052-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGM3 at approximately 63 kDa. The expected band size for PGM3 is at 60 kDa.
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