anti-Phospho-Histone H2B (Ser14), Rabbit Monoclonal (RM238)

RevMAb Biosciences

anti-Phospho-Histone H2B (Ser14), Rabbit Monoclonal (RM238)

5

This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.

Western Blot, ELISA, ImmunoCytoChemistry, Other Application

Research Use Only

Monoclonal

RM238

1 mg/ml

Liquid

H2BC3

H2B clustered histone 3

H2B histone family, member F; H2B.1; H2B/f; H2BFF; HIST1H2BB; histone 1, H2bb; histone cluster 1 H2B family member b; histone cluster 1, H2bb; histone H2B type 1-B; histone H2B.1; histone H2B.f

Rabbit

IgG

Histone H2B type 1-B

Histones are proteins that package DNA into nucleosomes. Histones are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA. There are five families of histones known to date; these histones are termed H1/H5, H2A, H2B, H3, and H4. H2B is considered a core histone, along with H2A, H3 and H4. H2A packages DNA molecules into chromatin and has been correlated with DNA modification and epigenetics. H2A plays a major role in determining the overall structure of chromatin and regulates gene expression. Protein modification on histone H2A exist and can sometimes result in a change in function. Different H2A variants were exploited to have different functions, genetic sequences and modifications. Histone H2B is a structural protein that helps organize eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. Hyperacetylation of histone tails helps DNA-binding proteins access chromatin by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of a specific lysine residue binds to bromine-containing domains of certain transcription and chromatin regulatory proteins. This docking facilitates the recruitment of these proteins to the correct region of the chromosome. Ubiquitinated histone H2B is often found in regions of active transcription and stimulates transcriptional elongation and sets the stage for further modifications that regulate multiple elements of transcription. In histone H2B, a phosphorylated serine or threonine residue activates transcription. - Recombinant Antibody. This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones. Applications: WB, ELISA, Multiplex, ICC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Histones are proteins that package DNA into nucleosomes. Histones are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA. There are five families of histones known to date; these histones are termed H1/H5, H2A, H2B, H3, and H4. H2B is considered a core histone, along with H2A, H3 and H4. H2A packages DNA molecules into chromatin and has been correlated with DNA modification and epigenetics. H2A plays a major role in determining the overall structure of chromatin and regulates gene expression. Protein modification on histone H2A exist and can sometimes result in a change in function. Different H2A variants were exploited to have different functions, genetic sequences and modifications. Histone H2B is a structural protein that helps organize eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. Hyperacetylation of histone tails helps DNA-binding proteins access chromatin by weakening histone-DNA and nucleosome-nucleosome interactions. Acetylation of a specific lysine residue binds to bromine-containing domains of certain transcription and chromatin regulatory proteins. This docking facilitates the recruitment of these proteins to the correct region of the chromosome. Ubiquitinated histone H2B is often found in regions of active transcription and stimulates transcriptional elongation and sets the stage for further modifications that regulate multiple elements of transcription. In histone H2B, a phosphorylated serine or threonine residue activates transcription.

Vertebrate

-20°C,2°C to 8°C

12352203