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ProductsBack/Anti-Phospho-Tie-2 (Y992) TEK Antibody
Western blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody
Western blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody
Western blot analysis of VEC cells using Phospho-Tie-2 (Y992) Polyclonal Antibody

Anti-Phospho-Tie-2 (Y992) TEK Antibody

A01274Y992
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTEK
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Phospho-Tie-2 (Y992) TEK Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID7010
  • Target name
    TEK
  • Target description
    TEK receptor tyrosine kinase
  • Target synonyms
    CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1, angiopoietin-1 receptor, TEK tyrosine kinase, endothelial, endothelial tyrosine kinase, tunica interna endothelial cell kinase, tyrosine kinase with Ig and EGF homology domains-2, tyrosine-protein kinase receptor TEK, tyrosine-protein kinase receptor TIE-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02763
  • Protein Name
    Angiopoietin-1 receptor
  • Scientific Description
    Boster Bio Anti-Phospho-Tie-2 (Y992) TEK Antibody catalog # A01274Y992. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TEK using anti-TEK antibody (A01274-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEK antigen affinity purified polyclonal antibody (Catalog # A01274-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEK at approximately 160KD. The expected band size for TEK is at 126KD.
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HUVEC cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ TIE2/CD202b Polyclonal Antibody(bs-1300R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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