Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using RevMAb Clone RM111 at 1/500 dilution (foll-owed by a PE conjugated secondary antibody, red) and DAPI (blue).
Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using RevMAb Clone RM111 at 1/500 dilution (foll-owed by a PE conjugated secondary antibody, red) and DAPI (blue).
anti-Phosphotyrosine, Rabbit Monoclonal (RM111)
REV-32-1011-00
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityAll Species
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-Phosphotyrosine, Rabbit Monoclonal (RM111)
- Delivery Days Customer5
- Antibody SpecificityThis antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM111
- Concentration1 mg/ml
- FormulationLiquid
- HostRabbit
- IsotypeIgG
- Scientific DescriptionRecombinant Antibody. This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine. Applications: WB, IP, ICC, IHC, FACS, ELISA . Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Tyrosine is a non-essential amino acid that is used by cells to synthesize proteins. Some of the tyrosine residues can be tagged (at the hydroxyl group) with a phosphate group (phosphorylated) by protein kinases. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine can be detected through specific antibodies. - Tyrosine is a non-essential amino acid that is used by cells to synthesize proteins. Some of the tyrosine residues can be tagged (at the hydroxyl group) with a phosphate group (phosphorylated) by protein kinases. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine can be detected through specific antibodies.
- ReactivityAll Species
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203