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Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.

Anti-PHYHD1 Antibody

HPA012115
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPHYHD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PHYHD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID254295
  • Target name
    PHYHD1
  • Target description
    phytanoyl-CoA dioxygenase domain containing 1
  • Target synonyms
    phytanoyl-CoA dioxygenase domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5SRE7
  • Protein Name
    Phytanoyl-CoA dioxygenase domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PHYHD1 using anti-PHYHD1 antibody (A12964-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHYHD1 antigen affinity purified polyclonal antibody (Catalog # A12964-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHYHD1 at approximately 32 kDa. The expected band size for PHYHD1 is at 32 kDa.
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