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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.

Anti-PHYHIPL Antibody

HPA038746
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPHYHIPL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PHYHIPL Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84457
  • Target name
    PHYHIPL
  • Target description
    phytanoyl-CoA 2-hydroxylase interacting protein like
  • Target synonyms
    phytanoyl-CoA hydroxylase-interacting protein-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FC7
  • Protein Name
    Phytanoyl-CoA hydroxylase-interacting protein-like
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PHYHIPL using anti-PHYHIPL antibody (A14122-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHYHIPL antigen affinity purified polyclonal antibody (Catalog # A14122-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHYHIPL at approximately 40 kDa. The expected band size for PHYHIPL is at 42,40,6 kDa.
Product group Antibodies
Boster Bio
Anti-PHYHIPL Antibody Picoband(r)A14122-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetPHYHIPL
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Atlas Antibodies
PrEST Antigen PHYHIPLAPREST80317
Protein IDQ96FC7
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Product group Antibodies
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PHYHIPL AntibodyCSB-PA017949GA01HU
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPHYHIPL
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PHYHIPL AntibodyLS-C754479
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