Western blot analysis of PIK3CD using anti-PIK3CD antibody on (1) K562 cell lysate; (2) RAW 264. cell lysate; (3) Rat kidney lysate. (M02269). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CD antigen affinity purified polyclonal antibody (Catalog # M02269) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Western blot analysis of PIK3CD using anti-PIK3CD antibody on (1) K562 cell lysate; (2) RAW 264. cell lysate; (3) Rat kidney lysate. (M02269). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CD antigen affinity purified polyclonal antibody (Catalog # M02269) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Anti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal Antibody
M02269
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-PI 3 Kinase p110 delta Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- FormulationLiquid
- Protein IDO00329
- Protein NamePhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
- Scientific DescriptionBoster Bio Anti-PI 3 Kinase p110 delta PIK3CD Rabbit Monoclonal Antibody catalog # M02269. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
- Storage Instruction-20°C
- UNSPSC12352203