Anti-PI-LPS [1E4]

Absolute Antibody

Anti-PI-LPS [1E4]

7

The antibody recognizes a PI specific epitope on PI-LPS. The antibody does not cross react with PII-LPS.

The specificity of the antibody (IgG2a) for PI-LPS was confirmed by ELISA analysis. The antibody detected PI-LPS by western blot analysis. The antibody was able to inhibit C. burnetii infection in vivo in a dose-dependent manner (Peng et al., 2012; PMID: 23053512). The original antibody could confer protection against C. burnetii aerosol infection. The Fab fragment, the scFv fragment and the humanized version of the antibody were constructed and characterized by indirect ELISA and Western Blot. They were able to bind to C. burnetii and to inhibit the infection in mice and in mouse Bone Marrow-Derived Macrophages (BMDM) in vitro. The humanized version inhibited C. burnetii infection in human macrophages in vitro. The Fab fragment was able to neutralize virulent C. burnetii resulting in inhibiting C. burnetii infection in both in vitro and in vivo systems (US20150087807A1). The ability of the original antibody, Fab, scFv, and humanized version of the antibody to inhibit C. burnetii infection in vivo was evaluated by comparing splenomegaly, bacterial burden, and pathological changes in the spleen with control mice at 14 days postinfection. IFA was used to analyze the ability of the original antibody to bind live virulent C. burnetii. The ability of the original to inhibit C. burnetii was higher than the ability of the other fragments. The humanized version of the antibody showed potential as a therapeutic against C. burnetii exposure (Peng et al., 2014; PMID: 25114119).

Western Blot, ELISA, Other Application

ELISA; WB; in vivo; in vitro

Research Use Only

Monoclonal

1E4

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Bacteria

C. burnetii

The original antibody was generated by immunizing BALB/c mice with formalin-inactivated C. burnetii Nine Mile PI antigen.

-20°C,2°C to 8°C

12352203