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Western blot analysis in human plasma.
Western blot analysis in human plasma.
Western blot analysis in human plasma.

Anti-PI16 Antibody

HPA043763
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPI16
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PI16
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID221476
  • Target name
    PI16
  • Target description
    peptidase inhibitor 16
  • Target synonyms
    CD364, CRISP9, MSMBBP, PSPBP, peptidase inhibitor 16, PSP94-binding protein, cysteine-rich secretory protein 9, microseminoprotein, beta-binding protein, protease inhibitor 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UXB8
  • Protein Name
    Peptidase inhibitor 16
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PI-16/PI16 using anti-PI-16/PI16 antibody (A09887-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: monkey heart tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI-16/PI16 antigen affinity purified polyclonal antibody (Catalog # A09887-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI-16/PI16 at approximately 70-75KD. The expected band size for PI-16/PI16 is at 70-75KD.
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