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Anti-PICALM (Center) Antibody

102-24632
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetPICALM
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PICALM (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB53756
  • Gene ID8301
  • Target name
    PICALM
  • Target description
    phosphatidylinositol binding clathrin assembly protein
  • Target synonyms
    CALM; clathrin assembly lymphoid myeloid leukemia protein; CLTH; LAP; phosphatidylinositol-binding clathrin assembly protein
  • Host
    Rabbit
  • Protein IDQ13492
  • Protein Name
    Phosphatidylinositol-binding clathrin assembly protein
  • Scientific Description
    Rabbit Anti-PICALM (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PICALM using anti-PICALM antibody (A02053-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PICALM antigen affinity purified polyclonal antibody (Catalog # A02053-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PICALM at approximately 71KD. The expected band size for PICALM is at 71KD.
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