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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-PID1 Antibody

HPA036103
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPID1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PID1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55022
  • Target name
    PID1
  • Target description
    phosphotyrosine interaction domain containing 1
  • Target synonyms
    HMFN2073, NYGGF4, P-CLI1, PCLI1, PTB-containing, cubilin and LRP1-interacting protein, phosphotyrosine interaction domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z2X4
  • Protein Name
    PTB-containing, cubilin and LRP1-interacting protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PID1 using anti-PID1 antibody (A07451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PID1 antigen affinity purified polyclonal antibody (Catalog # A07451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PID1 at approximately 28 kDa. The expected band size for PID1 is at 28 kDa.
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