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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Anti-PITHD1 Antibody

HPA016936
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPITHD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PITHD1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57095
  • Target name
    PITHD1
  • Target description
    PITH domain containing 1
  • Target synonyms
    C1orf128, HT014, TXNL1CL, PITH domain-containing protein 1, PITH (C-terminal proteasome-interacting domain of thioredoxin-like) domain containing 1, TXNL1 C-terminal like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZP4
  • Protein Name
    PITH domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PITHD1 using anti-PITHD1 antibody (A15347-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITHD1 antigen affinity purified polyclonal antibody (Catalog # A15347-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITHD1 at approximately 24 kDa. The expected band size for PITHD1 is at 24 kDa.
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TargetPITHD1
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