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Immunohistochemical staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferous ducts.

Anti-PIWIL4 Antibody

HPA036588
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPIWIL4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PIWIL4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID143689
  • Target name
    PIWIL4
  • Target description
    piwi like RNA-mediated gene silencing 4
  • Target synonyms
    HIWI2; MIWI2; piwi-like 4; piwi-like protein 4; testis tissue sperm-binding protein Li 85P
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3Z4
  • Protein Name
    Piwi-like protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA104445(PIWIL4 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
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Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA057508 antibody. Corresponding PIWIL4 RNA-seq data are presented for the same tissues.
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Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA057508 antibody. Corresponding PIWIL4 RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of PIWIL4/PIWI using anti-PIWIL4/PIWI antibody (A06797-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIWIL4/PIWI antigen affinity purified polyclonal antibody (Catalog # A06797-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIWIL4/PIWI at approximately 97 kDa. The expected band size for PIWIL4/PIWI is at 97 kDa.
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