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Immunohistochemical staining of human parathyroid gland shows moderate positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows moderate positivity in glandular cells.
Immunohistochemical staining of human parathyroid gland shows moderate positivity in glandular cells.

Anti-PLA2G4A Antibody

HPA054206
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLA2G4A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLA2G4A
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5321
  • Target name
    PLA2G4A
  • Target description
    phospholipase A2 group IVA
  • Target synonyms
    GURDP, PLA2G4, cPLA2, cPLA2-alpha, cytosolic phospholipase A2, calcium-dependent phospholipid-binding protein, lysophospholipase, phosphatidylcholine 2-acylhydrolase, phospholipase A2, group IVA (cytosolic, calcium-dependent)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP47712
  • Protein Name
    Cytosolic phospholipase A2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PLA2G4A using anti-PLA2G4A antibody (PB9778). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: NIH/3T3 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G4A antigen affinity purified polyclonal antibody (Catalog # PB9778) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G4A at approximately 85 kDa. The expected band size for PLA2G4A is at 85 kDa.
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