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Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in parietal cells.
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in parietal cells.
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in parietal cells.

Anti-PLAC9 Antibody

HPA043469
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLAC9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLAC9
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID219348
  • Target name
    PLAC9
  • Target description
    placenta associated 9
  • Target synonyms
    placenta-specific protein 9, placenta specific 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5JTB6
  • Protein Name
    Placenta-specific protein 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLAC9 using anti-PLAC9 antibody (A17853-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAC9 antigen affinity purified polyclonal antibody (Catalog # A17853-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAC9 at approximately 17KD. The expected band size for PLAC9 is at 10KD.
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