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Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.

Anti-PLAUR Antibody

HPA050843
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLAUR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLAUR Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5329
  • Target name
    PLAUR
  • Target description
    plasminogen activator, urokinase receptor
  • Target synonyms
    CD87, U-PAR, UPAR, URKR, urokinase plasminogen activator surface receptor, monocyte activation antigen Mo3, u-plasminogen activator receptor form 2, urokinase-type plasminogen activator (uPA) receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03405
  • Protein Name
    Urokinase plasminogen activator surface receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLAUR using anti-PLAUR antibody (A00993-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAUR antigen affinity purified polyclonal antibody (Catalog # A00993-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAUR at approximately 39KD, 55KD. The expected band size for PLAUR is at 37KD.
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GeneTex
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