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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Anti-PLBD2 Antibody

HPA017163
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPLBD2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLBD2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID196463
  • Target name
    PLBD2
  • Target description
    phospholipase B domain containing 2
  • Target synonyms
    76 kDa protein; LAMA-like protein 2; lamina ancestor homolog 2; mannose-6-phosphate protein associated protein p76; P76; phospholipase B domain-containing protein 2; phospholipase B-like 2 32 kDa form; phospholipase B-like 2 45 kDa form; PLB homolog 2; putative phospholipase B-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NHP8
  • Protein Name
    Putative phospholipase B-like 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLBD2 using anti-PLBD2 antibody (A12971). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLBD2 antigen affinity purified polyclonal antibody (Catalog # A12971) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLBD2 at approximately 75 kDa. The expected band size for PLBD2 is at 66 kDa.
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