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Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows  strong cytoplasmic positivity in macrophages.
Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.

Anti-PLCE1 Antibody

HPA015597
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLCE1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLCE1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51196
  • Target name
    PLCE1
  • Target description
    phospholipase C epsilon 1
  • Target synonyms
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1; NPHS3; pancreas-enriched phospholipase C; phosphoinositide phospholipase C; phosphoinositide phospholipase C-epsilon-1; phosphoinositide-specific phospholipase C epsilon-1; PLCE; PLC-epsilon-1; PPLC
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P212
  • Protein Name
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLCE1 using anti-PLCE1 antibody (A02360-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCE1 antigen affinity purified polyclonal antibody (Catalog # A02360-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLCE1 at approximately 290 kDa. The expected band size for PLCE1 is at 259 kDa.
Product group Antibodies
Boster Bio
Anti-PLCE1 Antibody Picoband(r)A02360-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetPLCE1
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ICC/IF analysis of PFA-fixed HeLa cells using GTX02580 PLCE1 antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:200
Product group Antibodies
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPLCE1
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Product group Antibodies
LifeSpan BioSciences
PLCE1 AntibodyLS-C663427
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
TargetPLCE1
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Atlas Antibodies
Protein IDQ9P212
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