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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Anti-PLD6 Antibody

HPA049345
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLD6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLD6 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID201164
  • Target name
    PLD6
  • Target description
    phospholipase D family member 6
  • Target synonyms
    ZUC, mitochondrial cardiolipin hydrolase, PLD 6, Zucchini endoribonuclease, choline phosphatase 6, mitoPLD, mitochondrial phospholipase, phosphatidylcholine-hydrolyzing phospholipase D6, phospholipase D6, protein zucchini homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N2A8
  • Protein Name
    Mitochondrial cardiolipin hydrolase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PLD6 using anti-PLD6 antibody (A10904-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLD6 antigen affinity purified polyclonal antibody (Catalog # A10904-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLD6 at approximately 28 kDa. The expected band size for PLD6 is at 28 kDa.
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