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Immunohistochemical staining of human lactating breast shows strong positivity in glandular cells.
Immunohistochemical staining of human lactating breast shows strong positivity in glandular cells.
Immunohistochemical staining of human lactating breast shows strong positivity in glandular cells.

Anti-PLIN2 Antibody

HPA016607
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLIN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLIN2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID123
  • Target name
    PLIN2
  • Target description
    perilipin 2
  • Target synonyms
    ADFP, ADRP, perilipin-2, adipophilin, adipose differentiation-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99541
  • Protein Name
    Perilipin-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ADFP using anti-ADFP antibody (PB10083). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADFP antigen affinity purified polyclonal antibody (Catalog # PB10083) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADFP at approximately 48 kDa. The expected band size for ADFP is at 48 kDa.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ADFP antibody [N1C2] (GTX110204) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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