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Western blot analysis in human cell line NTERA-2.
Western blot analysis in human cell line NTERA-2.
Western blot analysis in human cell line NTERA-2.

Anti-PLK1 Antibody

AMAB91515
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPLK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLK1
  • Delivery Days Customer
    4
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL8360
  • Conjugate
    Unconjugated
  • Gene ID5347
  • Target name
    PLK1
  • Target description
    polo like kinase 1
  • Target synonyms
    PLK, STPK13, serine/threonine-protein kinase PLK1, PLK-1, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, serine/threonine-protein kinase 13
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP53350
  • Protein Name
    Serine/threonine-protein kinase PLK1
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PLK1 using anti-PLK1 antibody (PB9500). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40ug, Lane 2: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLK1 antigen affinity purified polyclonal antibody (Catalog # PB9500) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLK1 at approximately 68 kDa. The expected band size for PLK1 is at 68 kDa.
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Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.
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