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Immunohistochemical staining of human duodenum shows strong membranous positivity in endothelial cells.
Immunohistochemical staining of human duodenum shows strong membranous positivity in endothelial cells.
Immunohistochemical staining of human duodenum shows strong membranous positivity in endothelial cells.

Anti-PLVAP Antibody

HPA002279
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPLVAP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PLVAP
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83483
  • Target name
    PLVAP
  • Target description
    plasmalemma vesicle associated protein
  • Target synonyms
    DIAR10, FELS, PV-1, PV1, gp68, plasmalemma vesicle-associated protein, fenestrated endothelial-linked structure protein, fenestrated-endothelial linked structure protein; PV-1 protein, plasmalemma vesicle protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BX97
  • Protein Name
    Plasmalemma vesicle-associated protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLVAP using anti-PLVAP antibody (A07024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLVAP antigen affinity purified polyclonal antibody (Catalog # A07024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLVAP at approximately 53 kDa. The expected band size for PLVAP is at 51 kDa.
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