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Immunohistochemical staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate to strong nuclear positivity in glandular cells.

Anti-POGK Antibody

HPA031630
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPOGK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POGK
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57645
  • Target name
    POGK
  • Target description
    pogo transposable element derived with KRAB domain
  • Target synonyms
    BASS2; KRAB box domain containing 2; KRBOX2; LST003; pogo transposable element with KRAB domain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P215
  • Protein Name
    Pogo transposable element with KRAB domain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POGK using anti-POGK antibody (A15311-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POGK antigen affinity purified polyclonal antibody (Catalog # A15311-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POGK at approximately 95 kDa. The expected band size for POGK is at 69 kDa.
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