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Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells and islets of Langerhans.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells and islets of Langerhans.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells and islets of Langerhans.

Anti-POLR1D Antibody

HPA039337
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPOLR1D
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POLR1D
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51082
  • Target name
    POLR1D
  • Target description
    RNA polymerase I and III subunit D
  • Target synonyms
    AC19; DNA-directed RNA polymerase I subunit D; DNA-directed RNA polymerases I and III subunit RPAC2; POLR1C; polymerase (RNA) I polypeptide D, 16kDa; polymerase (RNA) I subunit D; Protein POLR1D; RNA polymerase I subunit D; RNA polymerases I and III subunit AC2; RPA16; RPA9; RPAC2; RPC16; RPO1-3; TCS2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2S0
  • Protein Name
    Protein POLR1D, isoform 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLR1D using anti-POLR1D antibody (A07808-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1D antigen affinity purified polyclonal antibody (Catalog # A07808-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR1D at approximately 20 kDa. The expected band size for POLR1D is at 15 kDa.
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