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Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line Hep G2 shows localization to nuclear speckles.

Anti-POLR2D Antibody

HPA034694
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPOLR2D
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POLR2D
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5433
  • Target name
    POLR2D
  • Target description
    RNA polymerase II subunit D
  • Target synonyms
    DNA-directed RNA polymerase II 16 kDa polypeptide; DNA-directed RNA polymerase II subunit D; DNA-directed RNA polymerase II subunit RPB4; HSRBP4; HSRPB4; polymerase (RNA) II (DNA directed) polypeptide D; polymerase (RNA) II subunit D; RBP4; RNA polymerase II 16 kDa subunit; RNA polymerase II subunit B4; RNA polymerase II subunit hsRBP4; RNA polymerase II subunit hsRPB4; RPB16; RPB4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15514
  • Protein Name
    DNA-directed RNA polymerase II subunit RPB4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR2D using anti-POLR2D antibody (A12839-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2D antigen affinity purified polyclonal antibody (Catalog # A12839-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2D at approximately 18 kDa. The expected band size for POLR2D is at 16-20 kDa.
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