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Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Anti-POLR3GL Antibody

HPA027288
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOLR3GL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POLR3GL Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84265
  • Target name
    POLR3GL
  • Target description
    RNA polymerase III subunit GL
  • Target synonyms
    RPC32HOM, SOFM, flj32422, DNA-directed RNA polymerase III subunit RPC7-like, DNA-directed RNA polymerase III subunit G-like, RNA polymerase III 32 kDa beta subunit, RNA polymerase III subunit C7-like, RNA polymerase III subunit G like, RPC32-beta, RPC32-like protein, alternative RNA polymerase III subunit 32, polymerase (RNA) III (DNA directed) polypeptide G (32kD)-like, polymerase (RNA) III subunit G like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BT43
  • Protein Name
    DNA-directed RNA polymerase III subunit RPC7-like
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POLR3GL using anti-POLR3GL antibody (A17067-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR3GL antigen affinity purified polyclonal antibody (Catalog # A17067-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR3GL at approximately 24 kDa. The expected band size for POLR3GL is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-POLR3GL Antibody Picoband(r)A17067-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetPOLR3GL
  • SizePrice
Atlas Antibodies
PrEST Antigen POLR3GLAPREST76696
Protein IDQ9BT43
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
POLR3GL Antibody (aa65-93)LS-C163859
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPOLR3GL
  • SizePrice