Anti-Polysialic acid [735]

Absolute Antibody

Anti-Polysialic acid [735]

7

Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of alpha2,8-linked sialic acid of Escherichia coli K1.

Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989; PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000; PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).

Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

IHC; FC; IP

Research Use Only

Monoclonal

735

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse, Rat

Human, Mouse, Rat

Homopolymers of alfa2,8-linked sialic acid of Escherichia coli K1.

-20°C,2°C to 8°C

12352203