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Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.

Anti-POMGNT1 Antibody

HPA044518
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPOMGNT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POMGNT1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55624
  • Target name
    POMGNT1
  • Target description
    protein O-linked mannose N-acetylglucosaminyltransferase 1 (beta 1,2-)
  • Target synonyms
    GNTI.2, GnT I.2, LGMD2O, LGMDR15, MEB, MGAT1.2, RP76, gnT-I.2, protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1, UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WZA1
  • Protein Name
    Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of POMGNT1 using anti-POMGNT1 antibody (A03489-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMGNT1 antigen affinity purified polyclonal antibody (Catalog # A03489-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMGNT1 at approximately 75 kDa. The expected band size for POMGNT1 is at 75 kDa.
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