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Immunohistochemical staining of human fallopian tube shows moderate to strong positivity in cilia in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate to strong positivity in cilia in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate to strong positivity in cilia in glandular cells.

Anti-POMGNT2 Antibody

HPA040989
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPOMGNT2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POMGNT2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84892
  • Target name
    POMGNT2
  • Target description
    protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-)
  • Target synonyms
    AGO61; C3orf39; glycosyltransferase-like domain containing 2; glycosyltransferase-like domain-containing protein 2; GTDC2; MDDGA8; MDDGC8; protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2; protein O-mannose beta-1,4-N-acetylglucosaminyltransferase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NAT1
  • Protein Name
    Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POMGNT2 using anti-POMGNT2 antibody (A11987-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POMGNT2 antigen affinity purified polyclonal antibody (Catalog # A11987-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POMGNT2 at approximately 75 kDa. The expected band size for POMGNT2 is at 67 kDa.
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