Anti-PorA protein [207,B-4]

Absolute Antibody

Anti-PorA protein [207,B-4]

9

This antibody recognizes the P1.7 loop of the PorA protein of Neisseria meningitidis. Epitope mapping revealed that this antibody specifically binds a sequence ASGQ. The binding of this antibody was enhanced by the addition of a C-terminal valine residue

This antibody was reported to have bactericidal and opsonophagocytic effects for P1.7-expressing meningococci. This antibody demonstrated high opsonophagocytic activity with >80% of polymorphonuclear leukocytes (PMNs) exhibiting measurable respiratory burst (RB) at a concentration of the MAbs of <10 microg/ml. The phagocytic activity was tested against log-phase-growth strain 44/76-SL bacteria by using human serum as a complement source, human PMNs as effector cells, and increasing concentrations of the antibody as the opsonin. The test was performed by using flow cytometry to measure the respiratory burst induced in the effector cells following phagocytosis of the test strain. The epitope mapping of this antibody was done using ELISA (PMID: 10722576).

Flow Cytometry, ELISA

Research Use Only

Monoclonal

207,B-4

Rabbit

IgG

Major outer membrane protein P.IA

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203