Anti-PorA protein [MN12H2]

Absolute Antibody

Anti-PorA protein [MN12H2]

9

This antibody shows high bactericidal activity. This antibody binds the bacterial membrane surface protein resulting in activation of the classical complement pathway and subsequent lysis of the cell (PMID: 9880525). A study on antibacterial activity of human chimeric IgG1 (chIgG1) and chimeric IgG3 (chIgG3) version of this antibody showed that the serum bactericidal activity of chIgG1 was greater than that of chIgG3, and chIgG3 was better than chIgG1 in terms of opsonophagocytic (PMID: 11342648). This antibody provided protection in vivo against the prototype P1.7,16a, variably against the P1.7,16b and P1.7,16c, but not against the P1.7,16d variants in an infant rat infection model (PMID: 11273739). This antibody and the chimeric hIgG1 and hIgG3 derivatives all induced efficient bactericidal activity in vitro in the presence of human or infant rat complement and augmented bacterial clearance in complement-sufficient HsdBrlHan:WIST rats, while the hIgG4 was unable to do so. In C6-deficient PVG/c- rats, lacking complement-mediated bacterial lysis, the augmentation of bacterial clearance this antibody and chimeric hIgG1 version was impaired compared to that in the syngeneic complement-sufficient PVG/c+ rat strain (PMID: 16622217).

Functional Assay, ELISA

Research Use Only

Monoclonal

MN12H2

Mouse

IgG2a

Major outer membrane protein P.IA

Bacteria

-20°C,2°C to 8°C

41116161