Anti-PorA protein [MN14C11.6]

Absolute Antibody

Anti-PorA protein [MN14C11.6]

7

This antibody showed both bactericidal and opsonophagocytic for P1.7-expressing meningococci. The lowest concentration resulting in >50% killing of strain 44/76-SL was reported to be 0.5 microg/l. The opsonic activity of this antibody was measured against strain 44/76-SL with human peripheral blood polymorphonuclear leukocytes (PMN) as the effector cells and human serum as the complement source. This antibody demonstrated high opsonophagocytic activity with >80% of PMNs exhibiting measurable RB at a concentration of the MAbs of <10 microg/ml. This antibody was opsonic for the test strain 44/76-SL at a concentration of 0.2 microg/ml (PMID: 10722576). This antibody is not capable of binding meningococcal isolates expressing some variations of the P1.7 family especially P1.7b in a whole cell ELISA (PMID: 10512717). This antibody can be used in the detection of outer membrane protein of N. meningitidis strains like 44/76 using an ELISA (PMID: 12753502). This antibody was also used for the analysis of isogenic target strains derived from H44/76 (B:15:P1.7,16) in a western blot assay (PMID: 8698504).

Functional Assay, Western Blot, ELISA

ELISA; WB; functional assay

Research Use Only

Major outer membrane protein P.IA

-20°C,2°C to 8°C

12352203